Chemicals
Diesel oil used in this study was obtained from a local petrol
pump (Sfax, Tunisia). Diesel oil was used as a model contaminant
because it is a common environmental polluant. SDS (anionic surfactant)
and Tween 80 (non-ionic surfactant) were purchased from
Sigma and dissolved in distilled water. They were used as synthetic
chemical emulsifiers to perform the solubilization tests in parallel
with the biosurfactant from An6 strain.