2. Methods
2.1. Materials
Waste grease (21.7% of FFA) collected from grease traps in
Singapore’s sewage was donated by Alpha Synovate Pte Ltd.,
Singapore. Optimized genes of C. antarctica lipase B (CALB)
(Accession No. Z30645.1, NCBI) and T. lanuginosus lipase (TLL)
(Accession No. AF054513.1, NCBI) were purchased from GenScript
Singapore. Standard fatty acid methyl esters (FAME) were obtained
from Sigma. Novozyme 435 (immobilized C. antarctica lipase B on
acrylic resin) and Lipozyme TLIM (immobilized T. lanuginosus