Previous studies used fluorescence spectrophotometers to
measure the fluorescence. They usually used single wave-
length for the emission of fluorescence. However, since the
ambientlightisnotamonochromaticlight,theuseofUVcom-
ponent of the standard illuminant D65 has clinical relevance
[1]
. The difference in spectral reflectance in the present study
canberegardedasthefluorescenceofsubstanceexcitedbythe
UV component of the standard illuminant D65. Standard illu-
minantD65representsaveragedaylightwithacorrelatedcolor
temperature of approximately 6500K
[17]
. Illuminant D65 con-
tains a rather strong emission in the UV range. Illuminant D65
contains relative spectral power of 37–69% in the wavelength
range of 330–395nm
[18]
.
To induce fluorescence of dentine, UV light of 365nm (or
363.8nm) was generally employed
[6]
. Dentin showed blue flu-
orescence in a peak at 440
±
10nm
[6]
, or 430nm
[20]
when sin-
gle wavelength UV light was irradiated. With the same exper-
imental protocol used in the present study
[1]
, dentin showed
fluorescent peak at 440nm, and the subtraction spectrum (flu-
orescence spectrum) showed a wide band form, which was
similartothoseofpreviousstudies
[6,20]
.Valuesinsubtraction
spectrumofwhitestandardtileusedinthepresentstudywere
negligible (
−
0.03 to 0.06% in wavelengths longer than 410nm).
Therefore, the white tile of the present study did not emitted
fluorescence despite the fact that some white standard tiles
showed fluorescence
[21]
.
5. Conclusion
Fluorescent emission was detected after addition of FWA
into experimental resin composites. FWA concentration influ-
encedthefluorescentpeakheightandarea,butthermocycling
up to 1000 cycles did not influence the values. Fluorescence
peak height and area were influenced by the resin matrices
composed of Bis-GMA, UDMA and TEGDMA. FWA added with
the concentrations of 0.01 and 0.05% emitted higher fluores-
cence than those from commercial resin composites. There-
fore, FWA could be used to increase the fluorescent emission
of a resin composite
Previous studies used fluorescence spectrophotometers tomeasure the fluorescence. They usually used single wave-length for the emission of fluorescence. However, since theambientlightisnotamonochromaticlight,theuseofUVcom-ponent of the standard illuminant D65 has clinical relevance[1]. The difference in spectral reflectance in the present studycanberegardedasthefluorescenceofsubstanceexcitedbytheUV component of the standard illuminant D65. Standard illu-minantD65representsaveragedaylightwithacorrelatedcolortemperature of approximately 6500K[17]. Illuminant D65 con-tains a rather strong emission in the UV range. Illuminant D65contains relative spectral power of 37–69% in the wavelengthrange of 330–395nm[18].To induce fluorescence of dentine, UV light of 365nm (or363.8nm) was generally employed[6]. Dentin showed blue flu-orescence in a peak at 440±10nm[6], or 430nm[20]when sin-gle wavelength UV light was irradiated. With the same exper-imental protocol used in the present study[1], dentin showedfluorescent peak at 440nm, and the subtraction spectrum (flu-orescence spectrum) showed a wide band form, which wassimilartothoseofpreviousstudies[6,20].Valuesinsubtractionspectrumofwhitestandardtileusedinthepresentstudywerenegligible (−0.03 to 0.06% in wavelengths longer than 410nm).Therefore, the white tile of the present study did not emittedfluorescence despite the fact that some white standard tilesshowed fluorescence[21].5. ConclusionFluorescent emission was detected after addition of FWAinto experimental resin composites. FWA concentration influ-encedthefluorescentpeakheightandarea,butthermocyclingup to 1000 cycles did not influence the values. Fluorescencepeak height and area were influenced by the resin matricescomposed of Bis-GMA, UDMA and TEGDMA. FWA added withthe concentrations of 0.01 and 0.05% emitted higher fluores-cence than those from commercial resin composites. There-fore, FWA could be used to increase the fluorescent emissionof a resin composite
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