Our study developed a simple, rapid and low-cost detection sys- tem by means of an alternative amperometric sensor. The change in electrochemically active metabolites production from microor- ganisms could be monitored to calculate bacterial count (and Lis- teria monocytogenes ). SCC was detected by measuring the current signals resulting from the expression of LDH activity. The objec- tives were to evaluate the effectiveness of the electrochemical sensor for determination of the bacterial count