3 ml deionized water (DI) in a 50 ml sterile Falcon tube was blended for 10 s at medium speed in a Sorvall Omni Mixer model 17,150 115 volt 5 amp (Newtown, Connecticut, USA.) 2 ml 1% sodium dodecyl sulphate (SDS) in ethanol was added and contents blended 10 additional seconds at medium speed. Falcon tubes were centrifuged (Allegra 6R, Beckman Coulter