All tanks were equipped with linear aeration supplied by a PVC pipe (90 cm long, 20 mm diam- eter with 36 holes of 1 mm) to keep the solids generated during cultivation in suspension and maintain the level of dissolved oxy- gen in the water at the recommended concentration for L. vannamei larval cultivation (>5 mg L−1 ). The water temperature was kept con- stant, between 29 and 30 ◦ C using 100-W heaters connected to a thermostat.