Table 2.
Development of cotyledonary somatic embryos from the different explants (leaf, stem and corm) of the five crocus species cultured in the presence of the four different media. Each medium is supplemented with (2 mg/L NAA + 2 mg/L TDZ) and 100 mg/L ABA. Data were collected after five weeks of culturing.
Crocus species Explants Callus response for cotyledonary somatic embryos
MS medium
GB5 medium
LS medium
CHE’ medium
Average number of embryos formeda Percent frequency of embryos
formed Average number of embryos formeda Percent frequency of embryos formed Average number of embryos formeda Percent frequency of embryos formed Average number of embryos formeda Percent frequency of embryos formed
Crocus specious ssp. Specious Leaf 2.5±0.2b 36.6b 1.2±0.4b 13.3c 1.4±0.3c 36.6a 1.3±0.6dc 43.3a
Stem 1.1±0.4d 23.3e 1.0±0.3c 10.0d 1.5±0.7c 10.0e 1.0±0.4de 16.6g
Corm 1.5±0.2c 26.6d 1.0±0.3c 13.3c 1.3±0.6c 16.6d 1.1±0.4dc 30.0d
Crocus oliveri ssp. Oliveri Leaf 2.9±0.4a 46.6a 1.5±0.2a 16.6b 1.3±0.6c 33.3b 1.8 ±0.4a 40.0b
Stem 1.5±0.2c 30.0c 1.1±0.1cb 20.0a 1.8±0.4b 16.6d 1.4±0.3bc 23.3e
Corm 1.8±0.4c 36.6b 1.1±0.1cb 16.6b 2.0±0.3a 26.6c 1.7±0.7ab 33.3c
Crocus pestalozzae Leaf 2.0±0.3b 26.6d 0.6±0.3d 13.3c 0.7±0.3d 16.6d 1.0±0.3dc 23.3e
Stem 0.7±0.3d 6.6g 0.2±0.3e 3.3f 0.2±0.3e 3.3g 0.4±0.2e 10.0h
Corm 0.9±0.6d 13.3f 0.3±0.3e 6.6e 0.4±0.2e 6.6f 0.6±0.3e 20.0f
Crocus abantensis Leaf – – – – – – – –
Stem – – – – – – – –
Corm – – – – – – – –
Crocus paschei(C. abantensis×C. oliveri ssp. Oliveri) Leaf – – – – – – – –
Stem – – – – – – – –
Corm – – – – – – –