The present study indicates that application of ultrasound waves (33 kHz) on strawberry. The ultrasound treatment helped in better retention of physicochemical parameters like TSS, tritrable acidity, pH and color of the sample during its storage period for 15 days at refrigerated temperature. The treatment not only maintained the firmness of the fruit, but also enhanced antioxidant activity and reduced the microbial load. All the parameters studied were found to be commercially more acceptable when the treatment time was limited to 30–40 min. The 30–40 min treatment time extended the shelf-life up to 15 days, where 0, 10 & 60 and 20 min of US treatment limited the shelf-life up to 6, 9 and 12 days, respectively. The higher treatment time showed detrimental effect on all the parameters. It was concluded that ultrasonic waves can be used as a minimal processing technique to enhance the shelf-life and consumer acceptability of perishable fruits. The future work should be carried out to explore its application on other perishable industrial crops and food products.