use an ethanol/lipids
molar ratio of 12:1 (equivalent to 4:1 alcohol/FFA), in the
transesterification of the lipids from the cyanobaterium
Microcystis aeruginosa, catalysed by Novozym 435, although these
authors used tert-butanol or iso-octane to preserve the stability
of the lipase. On the other hand, the low methanol/FFA ratio used
in this work contrasts with the high ratios that are required for
the production of biodiesel by esterification of FFAs by acid
catalysis.