The working range of soyasaponin I was defined to be linear
between 0.125 mg/mL and 0.625 mg/mL with a suitable average
coefficient of determination R2 = 0.993 ± 0.002. The limit of detection
(LOD) and limit of quantification (LOQ) were calculated to be 0.06 ±
0.01 mg/mL and 0.21 ± 0.01 mg/mL respectively. Furthermore, the
analysis revealed a recovery rate of 90 ± 4%. The measuring precision
within (repeatability) and between (reproducibility) batches was
obtained for soyasaponin I standard (c = 0.125–0.625 mg/mL) as well
as for the variety ‘Rocket’ (hulls). The relative standard deviation within
batches of soyasaponin I standard and in the pea sample did not exceed
9%. In contrast, the reproducibility of the measurement revealed a
relative standard deviation of maximum 12% (standard) and 11%
(sample).