To initiate the D. tertiolecta cultures a starter pre-culturewas used
to reach an initial concentration of 106 cell/ml of medium. Cultures
were set up in 250 ml Erlenmeyer flasks containing 150ml medium
at 25 C ± 2 C under continuous illumination at 150 mmol m2 s1.
Agitation of culture was 130 rpm in an orbital shaker