During the summer of 2003, sample units of cheese (regular, thick, and thin crust) and pepperoni (regular and thick crust) pizzas were collected from the top two national fast food pizza chains in 12 statistically selected locations in the 48 conterminous states. The fast food pizza chain locations were indentified using a multistage, stratified sampling plan developed for NFNAP [7]. Samples of both leading brands were again collected in October 2010. Sample units of the same types of fast food pizzas were collected from the pizza chains in the same statistically selected locations. Specialized protocols were followed to handle and ship sample pizza units to the Food Analysis Laboratory Control Center at Virginia Polytechnic Institute [8].