3.3.2. Standardization of conditions for efficient degreasing
Bacillus subtilis lipase carried out successful degreasing (>95%)
of goat and sheep skin and buffalo hides with 5e10% lipase in 12 h
of application without the use of any chemicals (Table 3, Fig. 4E, F).
Concentrated lipase having 142 IU/mL when applied on the flesh
side for approximately 12 h, there was a noticeable decrease in
weight of the goat, sheep skins and buffalo hide resulting in 961,
926 and 914 g from the initial weight of 995 g, 997 g and 993 g
respectively. This indicates successful removal of 34, 67 and 83 g
of fat from the goat, buffalo and sheep skins respectively after
treatment with this lipase.