A series of experimentswere conductedwith black tiger shrimp (Penaeusmonodon) juveniles to firstly determine
the effects of reducing fishmeal inclusion in a diet and then to evaluate the potential for a microbial bioactive to
support complete replacement of both fishmeal and fish oil in feedswhen fed under clear-water and green-water
conditions. The isoproteic and isoenergetic replacement of fishmeal resulted in a consistent decline in growth
performance indicating that at every decrease in fishmeal below an inclusion level of 45% there was a decline
in performance. In a subsequent trial undertaken in a clear-water tank system diets devoid of both fishmeal
and fish oil fed to shrimp were demonstrated to produce poorer performance than a fishmeal and fish oil reference
diet. However the addition of a microbial bioactive to the diet resulted in not only a compensation for the
replacement of these ingredients but also additional growth. Replication of the clear-water trial in a greenwater
tank system not only produced similar results, but also showed that the green-water system largely compensated
for the performance lost through replacement of fishmeal and fish oil. However it was also shown that
the use of themicrobial bioactive in the diets still resulted in improved growth performance of shrimp. This study
has effectively demonstrated a viable strategy for not only a complete replacement of all fishery products in
shrimp diets, but also an improved performance strategy.