After each transesterification reaction, the lipase was recovered
by filtration and washed with, in all cases, n-hexane, a tested
non-polar solvent, that causes greater retention of lipase activity
than that obtained when washing with the polar solvents, as we
previously we had already reported using others supports and other
alcohols. With all six alcohols that work as acyl acceptors, a high initial
yield was achieved. The lipase exhibited better activity during
the repeated experiments compared with those in the literature. In
the reaction with methanol, the production of alkyl esters was 8%
(determined using internal standards in GC); is of highlighting that
was detected production of biodiesel, new result obtained with this
system and has not been reported in the literature. The same experimental
conditions in the literature have not reported production of
biodiesel.