Fig. 3. Optimal pH (A), pH stability (B), optimal temperature (C), thermalstability (D) and thermal inactivation (E) of PbChi70 from P. barengoltzii . Symbols for optimal pH and
pH stability: (}) citrate buffer (pH 3.0–6.0); (d ) acetate buffer (pH 4.0–5.5); ( j) MES buffer (pH 5.5–6.5); (N) phosphate buffer (pH 6.0–8.0); (4) Tris–HCl buffer (pH 7.0–9.0);
(s) glycine-NaOH buffer (pH 9.0–10.5); ( ) phosphate-NaOH buffer (pH 10.5–12.0). Symbols for thermal inactivation: (N)55 C; (d)60 C; (4)65 C.