2.3. Grinding
Barley grain (10 g) for each genotype was ground to a fine powder
using an IKA Mill (Germany) with running water to avoid heat
increases during the milling.
2.4. Moisture content
The moisture content of barley flour was determined in triplicate
according to the procedure described in American
Association of Cereal Chemists-AACC (2000) Method No. 44-15A.
Two grams of sample was weighed into a pre-weighed dish and
dried in an air forced oven at a temperature of 105 ± 5 C until
the weight was constant.