A novel ligninolytic peroxidase gene (ACLnP) was cloned and characterized from a poroid brownrot
fungus, Antrodia cinnamomea. The genomic DNA of the fungus harboured two copies of
ACLnP, with a length of 2111 bp, interlaced with 12 introns, while the full-length cDNA was
1183 bp, with a 66 bp signal peptide and an ORF of 990 bp. The three-dimensional molecular
structure model was comparable to that of the versatile peroxidase of Pleurotus eryngii. ACLnP was
cloned into vector pQE31, successfully expressed in Escherichia coli strain M15 under the control
of the T5 promoter and produced a non-glycosylated protein of about 38 kDa, pI 5.42. The native
and recombinant ACLnP was capable of oxidizing the redox mediator veratryl alcohol, and also
decolorized bromophenol blue and 2,6-dimethoxyphenol dyes, implicating a functional extracellular
peroxidase activity. The significance of discovering a functional ACLnP gene in A. cinnamomea in
terms of wood degradation and colonization capacity in its unique niche is discussed
A novel ligninolytic peroxidase gene (ACLnP) was cloned and characterized from a poroid brownrot
fungus, Antrodia cinnamomea. The genomic DNA of the fungus harboured two copies of
ACLnP, with a length of 2111 bp, interlaced with 12 introns, while the full-length cDNA was
1183 bp, with a 66 bp signal peptide and an ORF of 990 bp. The three-dimensional molecular
structure model was comparable to that of the versatile peroxidase of Pleurotus eryngii. ACLnP was
cloned into vector pQE31, successfully expressed in Escherichia coli strain M15 under the control
of the T5 promoter and produced a non-glycosylated protein of about 38 kDa, pI 5.42. The native
and recombinant ACLnP was capable of oxidizing the redox mediator veratryl alcohol, and also
decolorized bromophenol blue and 2,6-dimethoxyphenol dyes, implicating a functional extracellular
peroxidase activity. The significance of discovering a functional ACLnP gene in A. cinnamomea in
terms of wood degradation and colonization capacity in its unique niche is discussed
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