Infection with the heat-killed spores was significantly less than the living spores. However, compared to the control group, heat-killed spores
resulted in observable larval killing (P = 0.000531) with 70 % larvae surviving the 168 h treatment (Fig. 2).
After 96 h of inoculation with the 42,000 living spores, 70 % of the larvae survived. The number of
surviving larvae dropped to 30 % in 120 h and to 20 % after 168 h of spore injection. Injecting larvae with the
heat-killed spores of A. terreus resulted in prolonged survival (P = 0.072) and 86 % larvae survived after 168
h of injection (Fig. 3). The strains A. niger (Gb) and A. niger (IMI60286) were tested for their pathogenicity.
The inoculum size injected was approximately 11,000 viable spores for A. niger (Gb) and around 39,000
living spores for (IMI60286). After 72 h of infection, no deaths were recorded for either strain. However by
the end of the 168 h incubation period, the strains exhibited different results with respect to pathogenicity (Fig.
4). A. niger (Gb) was clearly non-pathogenic (P = 0.513) with 93 % surviving larvae after 168 h of
inoculation. Although 80 % larvae survived by the end of experiment but statistical analyses showed larval
mortality by A. niger strain IMI60286 was significant (P = 0.00659).
Infection with the heat-killed spores was significantly less than the living spores. However, compared to the control group, heat-killed spores resulted in observable larval killing (P = 0.000531) with 70 % larvae surviving the 168 h treatment (Fig. 2). After 96 h of inoculation with the 42,000 living spores, 70 % of the larvae survived. The number of surviving larvae dropped to 30 % in 120 h and to 20 % after 168 h of spore injection. Injecting larvae with the heat-killed spores of A. terreus resulted in prolonged survival (P = 0.072) and 86 % larvae survived after 168 h of injection (Fig. 3). The strains A. niger (Gb) and A. niger (IMI60286) were tested for their pathogenicity. The inoculum size injected was approximately 11,000 viable spores for A. niger (Gb) and around 39,000 living spores for (IMI60286). After 72 h of infection, no deaths were recorded for either strain. However by the end of the 168 h incubation period, the strains exhibited different results with respect to pathogenicity (Fig. 4). A. niger (Gb) was clearly non-pathogenic (P = 0.513) with 93 % surviving larvae after 168 h of inoculation. Although 80 % larvae survived by the end of experiment but statistical analyses showed larval mortality by A. niger strain IMI60286 was significant (P = 0.00659).
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Infection with the heat-killed spores was significantly less than the living spores. However, compared to the control group, heat-killed spores
resulted in observable larval killing (P = 0.000531) with 70 % larvae surviving the 168 h treatment (Fig. 2).
After 96 h of inoculation with the 42,000 living spores, 70 % of the larvae survived. The number of
surviving larvae dropped to 30 % in 120 h and to 20 % after 168 h of spore injection. Injecting larvae with the
heat-killed spores of A. terreus resulted in prolonged survival (P = 0.072) and 86 % larvae survived after 168
h of injection (Fig. 3). The strains A. niger (Gb) and A. niger (IMI60286) were tested for their pathogenicity.
The inoculum size injected was approximately 11,000 viable spores for A. niger (Gb) and around 39,000
living spores for (IMI60286). After 72 h of infection, no deaths were recorded for either strain. However by
the end of the 168 h incubation period, the strains exhibited different results with respect to pathogenicity (Fig.
4). A. niger (Gb) was clearly non-pathogenic (P = 0.513) with 93 % surviving larvae after 168 h of
inoculation. Although 80 % larvae survived by the end of experiment but statistical analyses showed larval
mortality by A. niger strain IMI60286 was significant (P = 0.00659).
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