For this study, we isolated 28 isolates of locally sourced biobutanol-producing Clostridia. Among the 28 solvent-producing
cultures isolated, only one culture was selected for its ability to produce the highest concentrations of biobutanol as indicated by gas chromatography analysis. This isolate was identified as Clostridium acetobutylicum YM1 from the soil of a paddy field managed under a system of rice intensification (SRI).