Technologies, Waldbronn, Germany) equipped with a reversed
phase analytical polymeric C30 column (4.6 lm id. 3 mm
250 mm) (YMC, Inc. Wilmington, NC, USA) and a UV diode array
detector (set to 475 nm). The mobile phase of methyl t-butyl ether
/methanol/ ethyl acetate (40:50:10, v/v/v) was used at a flow of
2 mL/min. A sample volume of 20 lL was injected into the column.
The column temperature was maintained at 25 C. Analysis was
performed under dim light to prevent sample degradation by
photo-oxidation. The peak for trans-lycopene in the extract was
identified by comparing the retention time with that of a standard
compound, and the cis-isomers was estimated according to Nguyen
and Schwartz (1998).
Technologies, Waldbronn, Germany) equipped with a reversed
phase analytical polymeric C30 column (4.6 lm id. 3 mm
250 mm) (YMC, Inc. Wilmington, NC, USA) and a UV diode array
detector (set to 475 nm). The mobile phase of methyl t-butyl ether
/methanol/ ethyl acetate (40:50:10, v/v/v) was used at a flow of
2 mL/min. A sample volume of 20 lL was injected into the column.
The column temperature was maintained at 25 C. Analysis was
performed under dim light to prevent sample degradation by
photo-oxidation. The peak for trans-lycopene in the extract was
identified by comparing the retention time with that of a standard
compound, and the cis-isomers was estimated according to Nguyen
and Schwartz (1998).
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