cooling to room temperature, and the extract was centrifuged at
10000g, for 10 min, at 4 °C. This supernatant was then loaded into
200 mg Oasis HLB cartridges, previously conditioned with 5 mL of
water and 5 mL of methanol. The cartridges were then washed with 5
mL of water and eluted with 10 mL of methanol/ethyl acetate (50:50).
Finally, the eluate was evaporated to dryness under a gentle stream of
nitrogen, at 37 °C, and the dried extracts were analyzed.