2.2. Target Preparation and Validation of Docking Method
The three dimensional structure of glycogen phosphorylase was obtained from Brookhaven protein databank
(PDB ID: 1LWO). The docking study was started with the definition of a binding site, in general a restricted region
of the protein. The size and location of this binding site was visualized in PyMOL. The protein target were further
validated with AutoDock Vina in PyRx 0.8 by RMSD value determination8
.