Major implications on a country's economy, food source, and public health with recent concern over the highly pathogenic ND, avian influenza and FC outbreaks around the world, government agencies are carefully monitoring and inspecting live bird markets, commercial flocks, and migratory bird populations. However, there remains limited surveillance of non-commercial poultry. Therefore, a cross-sectional study with the useful application will conduct in backyard poultry flocks by using a convenience sampling method across 15 Tambon of Chiang Mai province of Thailand.