2.2. Preparation of coating mixture
Sodium alginate solution was prepared by dissolving 20 g in 1 L of
sterile distilled water at 70 °C. After 30 min stirring for complete dissolution,
glycerolwas added at 1.2 g/g alginate as a plasticizer,while CaCl2
(0.05 g/g alginate) was added to strengthen the coating structure. For
CBO coating mixtures, CBO was mixed with polysorbate 80 (Tween®
80) at a 4:1 mass ratio, followed by mixing with the alginate solution
with and without SBO on a magnetic stir plate. Water was added to
bring the total volume to 2 L. The final coating formulations contained
1% w/v alginate, 0.5% w/v Tween® 80, 0 or 2% w/v CBO, and 0 or
0.5% w/v SBO.