500Fig. 6. CCC-UV–vis chromatogram (450 nm) of the separation of crude carrot extractwith dual-mode utilization (mobile phase and elution mode reversal). Conditions:CCC-1000 with three serially connected multilayer coils (1.6 mm i.d. PTFE tubing)and a total column capacity of 325 mL and 10 mL sample loop; rotation speed:1010 ± 10 rpm; solvent system: n-hexane/benzotrifluoride/acetonitrile (10:3.5:6.5,v/v/v); sample size: 100.2 mg; separation: dual mode CCC separation with tail-to-head mode for 140 min at a flow rate of 2 mL/min and head-to-tail elution for 60 minat a flow rate of 4 mL/min