Assessment of the role of T. viride to enhance disease
resistance in legumes against plant pathogens
Seeds of Cajanus cajan (L.) var LRG 41, Vigna radiata var
LGG 460, Vigna mungo LBG 623 were surface sterilized with
ethanol (70% v/v) for 5 min, followed by mercuric chloride
(0.1%) for 4 min and rinsed five times with sterile distilled
water. Sterilized seeds were left overnight for soaking in sterile
distilled water. The overnight soaked seeds were pretreated
with T. viride spore suspension (106conidia/ml) for 30 min, blot
dried and sown in seedling trays (70 plugs), with each plug
measuring 4×4.5×2.5 cm. Equal number of seeds were sown as
controls without pretreatment with T. viride. The seeds were
left for germination and growth for one week. One week old
plants with and without pretreatment with T. viride were
exposed to F. oxysporum spores (with 106conidia/ml at the root
of the plant) and A. alternata spores (with 106 conidia/ml on
plant) and observations were noted down. Seeds without
pretreatment with T. viride which were exposed to F.
oxysporum and A. alternata are observed to assess the
interactions between host legumes and plant pathogens. The
legume seeds pretreated with T. viride followed by exposure to
F. oxysporum and A. alternata are observed for the role of T.
viride to enhance disease resistance in legumes against plant
pathogens. VI and DI; antioxidant enzymes and scavenging
activity of ROS by antioxidant enzymes and defense enzymes
are the parameters used to assess the interactions between host
legumes and plant pathogens and the role of T. viride to
enhance disease resistance in legumes against plant pathogens.