Detection and Quantification of IAA
Capability of endophytic fungi in producing IAA hormones was done using the method
used by Bhagobathi and Joshi (2009)[13] . Supernatan of endophytic fungi mixed with
Salkowsky reagent then incubated for 20 minutes. Observation of the color change before
absorbance was measured using a spectrophotometer with a wavelength of 535 nm. IAA
concentration of each isolate was compared to a standard curve.