2.5. Statistical analysis
Statistical analysis allowing a comparison of the content of antioxidant compounds was carried out using single-factor analysis of variance (ANOVA) on the basis of the Snedecor F and Student’s t tests, and the least significant difference (LSD) was calculated at the probability level p < 0.01. The Stastica 6.1 program was used.
3. Results and discussion
3.1. Effect of blanching and cooking kale leaves on the level of vitamin C, polyphenol constituents and antioxidative activity
Fresh kale leaves contained 112.1 mg vitamin C and 384.9 mg total identified polyphenols, with antioxidative activity of 1175 μM Trolox/100 g fresh matter (Table 1). Of the polyphenols identified in kale leaves, ferulic acid occurred in the highest amount (240.44 mg/100 g, constituting 62% of total polyphenols). The content of kaempferol (59.64 mg/100 g) and caffeic acid (41.54 mg/100 g) was considerably lower, comprising 15% and 11% of polyphenols respectively. The content of quercetin, p-coumaric acid and sinapinic acid constituted less than 5% of total polyphenols.
2.5. Statistical analysis
Statistical analysis allowing a comparison of the content of antioxidant compounds was carried out using single-factor analysis of variance (ANOVA) on the basis of the Snedecor F and Student’s t tests, and the least significant difference (LSD) was calculated at the probability level p < 0.01. The Stastica 6.1 program was used.
3. Results and discussion
3.1. Effect of blanching and cooking kale leaves on the level of vitamin C, polyphenol constituents and antioxidative activity
Fresh kale leaves contained 112.1 mg vitamin C and 384.9 mg total identified polyphenols, with antioxidative activity of 1175 μM Trolox/100 g fresh matter (Table 1). Of the polyphenols identified in kale leaves, ferulic acid occurred in the highest amount (240.44 mg/100 g, constituting 62% of total polyphenols). The content of kaempferol (59.64 mg/100 g) and caffeic acid (41.54 mg/100 g) was considerably lower, comprising 15% and 11% of polyphenols respectively. The content of quercetin, p-coumaric acid and sinapinic acid constituted less than 5% of total polyphenols.
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2.5. Statistical analysis
Statistical analysis allowing a comparison of the content of antioxidant compounds was carried out using single-factor analysis of variance (ANOVA) on the basis of the Snedecor F and Student’s t tests, and the least significant difference (LSD) was calculated at the probability level p < 0.01. The Stastica 6.1 program was used.
3. Results and discussion
3.1. Effect of blanching and cooking kale leaves on the level of vitamin C, polyphenol constituents and antioxidative activity
Fresh kale leaves contained 112.1 mg vitamin C and 384.9 mg total identified polyphenols, with antioxidative activity of 1175 μM Trolox/100 g fresh matter (Table 1). Of the polyphenols identified in kale leaves, ferulic acid occurred in the highest amount (240.44 mg/100 g, constituting 62% of total polyphenols). The content of kaempferol (59.64 mg/100 g) and caffeic acid (41.54 mg/100 g) was considerably lower, comprising 15% and 11% of polyphenols respectively. The content of quercetin, p-coumaric acid and sinapinic acid constituted less than 5% of total polyphenols.
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