To determine the regeneration ability, the initiated
calli were transferred to a regeneration medium.
Following transfer of the calli onto regeneration media,
shoot and root axis began to develop within 2–3 weeks
(Fig. 1-B). Regenerated shoots were sub-cultured after
every two weeks (Fig. 1-C, -D). We compared plant
regeneration ability among calli obtained from four
callus induction medium. It was observed that the
regeneration frequency was greatly influenced by
medium used for callus induction with the best results
(83.2%) achieved using the MS medium supplemented
with 2.0 mg/L 2,4-D, 500 mg/L proline and 500 mg/L
glutamine (Table 4).