This analyte, the riboflavin, will be studied by analyzing the emission spectrum out of the spectrophotometer. It should be noted that riboflavin must be reacted with acetic acid to become fluorescent. Another important aspect of fluorescence spectrophotometry is that the detector is at a right angle to the ultraviolet light used to excite the compound’s electrons. This is done so that the light source does not affect the fluorescence reading, as the detector cannot distinguish the source’s light from the analyte’s emission. With the readings, there will be a calibration curve created by the fluorescence reading against the concentration of riboflavin. This curve is very similar to a calibration curve of absorbance vs concentration, where it should show a positive linear relationship. This curve allows us to study different concentrations of riboflavin.
Results and Discussion