2.2 สัตว์และเงื่อนไขการทดลอง
Sexually immature common dentex (D. dentex; Actinopterygii,Sparidae) were raised at the Marine Culture Experimental Facilities of the Spanish Institute of Oceanography in Mazarrón (Murcia, Spain).
Fish were randomly selected and distributed into four triplicate groups of 24 fish each (91.7±1.4 g mean mass).
Each group was maintained at 18.0±1.0 °C, in 500-L tanks continuously supplied with seawater (37‰) at a 15-L min−1 flow rate.
Oxygen content was N85% saturation level.
The photoperiod was regulated as a 12:12 dark/light cycle.
After one-week acclimation, the experimental diets were randomly assigned to triplicate groups of animals that were hand-fed to apparent visual satiation, three times a day, for thirteen weeks.
Food intake and mortality were recorded daily and fish in each tank were bulk weighed
at the beginning and end of the experimental period.