Enzyme extraction was mixed with an equal volume of 0.5% polygalacturonic acid in 50 mmol L1 sodium acetate buffer (pH 5.5) with the presence of Ca2+, and incubated at 37 C
Enzyme extraction was mixed with an equal volumeof 0.5% polygalacturonic acid in 50 mmol L1 sodium acetatebuffer (pH 5.5) with the presence of Ca2+, and incubated at 37 C