The repeatability and reproducibility of the TMS1 gene quantitative
PCR assay were both evaluated using the same set of papaya
genomic DNA dilutions used to construct the standard curves. The
standard deviation (SDr) and relative standard deviation (RSDr) of
repeatability reflected the variation in the results at each DNA dilution
level. All of those values were calculated from three repeats
and each repeat with three replicates according to ISO 5725. The
RSDr of the quantitative PCR assay of the TMS1 gene ranged from
3.54% to 6.9% indicating that the established quantitative PCR
assay of the Agrobacterium sp. TMS1 gene is stable and reliable
(Table 3).