AG-CH NPs were prepared with the same CH/AG charge ratio (n+/n− = 1:10) but using different Ls:AG ratios, aiming to optimize the loading. The above charge ratio was chosen due to the good particle stability and properties reported elsewhere.18 Table 1 shows the characteristics and related properties of the AG-CH NPs. Due to the fact that the samples were isolated by spray drying, recovery yields varied from 30.4 to 61.1%, resulting in an average yield of 50%, which can be considered satisfactory. On decreasing the Ls:AG ratio from 1:2 to 1:20 the encapsulation efficiency increases, reaching a maximum at 77.8%. The Ls:AG-CH 1:20 sample exhibits a lower Ls loading value and a satisfactory encapsulation efficiency (around 70%). It can be concluded that the sample with Ls:AG-CH 1:10 presents the best parameters for the biolarvicide encapsulation by AG-CH NPs, with 6.5% of loading and 77.8% of encapsulation efficiency. These values are in agreement with those found by Fernandes et al.,25 where the encapsulation of Ls by cyclodextrin resulted in loading values of 5.6%. Other authors have reported similar loading values for the encapsulation of pesticides using biopolymers as matrices, with microspheres based on cellulose derivatives loaded with a commercial herbicide presented loading values in the range of 8-11%,26 while alginate beads loaded with a natural pesticide Neem (Azadirachta Indica A. Juss) showed loading values of 10 to 30%.21