Tonsil scraping samples were then collected from the steel spoon using a 15 cm sterile nylon flocked swab (Puritan1) and placed in 5 ml tubes containing 3 ml of sterile PBS. All samples were stored at 20 8C until assayed by PCR.
Tonsil scrapingsamples were then collected from the steel spoon using a15 cm sterile nylon flocked swab (Puritan1) and placed in5 ml tubes containing 3 ml of sterile PBS. All samples werestored at 20 8C until assayed by PCR.