Pycnogenol1, a standardized extract of the bark of the French Pinus Pinaster, has been used worldwide as an herbal
remedy and nutritional supplement in many kinds of degenerative diseases.12–14 Pycnogenol1 can be used in order
to ameliorate excessive oxidative stress in several cell systems by doubling the intracellular synthesis of anti-oxidative
enzymes. Recently, a dentifrice containing Pycnogenol1,anti-inflammatory and anti-bacterial agents (Tomarina1,
Kobayashi Pharmaceutical Co., Ltd., Osaka) was developed to attenuate the effects of ageing on periodontal tissue.
However, it is unclear how Tomarina1 affects periodontal tissue changes on ageing. Therefore, the purpose of the
present study was to examine the anti-ageing effects of topical application of Tomarina1 on gingival collagen density and
alveolar bone resorption in the rat model.