. Although the presence of two cytological entities in Algonquin Park has long been recognized (e.g. Basrur & Rothfels 1959), the presence of three deeply divergent branches suggests that a third species might be represented in Algonquin Park. Intriguingly, Currie & Hunter (2003) suggested that St. mutatawas the product of hybridization between St. diplomutataand a yet unidentified diploid species of Stegopterna. Whether one of the divergent branches of the St. mutatacomplex from Algonquin Park represents this unknown diploid species requires cytological confirmation. Nonetheless, DNA barcoding strongly suggests the presence of three species of Stegopternain Algonquin Park where only two such species were known previously.