2.5. Estimation of carbohydrates
Freeze-dried material of the mushroom fruiting bodies,
grown on cotton seed supplemented rice straw, was
subjected to extraction in 70% alcohol and the free and
total sugars determined by the phenol–sulphuric acid
method (Dubois, Gilles, Hamilton, Rebers, & Smith,
1956).
The alcohol – insoluble residue was hydrolyzed using
72% sulphuric acid and the total sugars estimated, as
above, in the hydrolysate, represented the polysaccharide
(alcohol – insoluble carbohydrates) (Rajarathnam
et al., 1979)