The objective of this study was to isolate and identify yeast strains from broilers excreta and to evaluate
in vitro their potential for probiotic use in animal production.
Methods and results: Nine yeast strains were isolated and presumptively pre-identified by biochemical
assays. These isolates were grouped in six different molecular profiles using PCR-fingerprinting technique.
Each profile was identified by sequencing of the D1/D2 domains of the large subunit of the 26S
rRNA gene. These yeasts were identified as: Trichosporon sp. (LV-2), Wickerhamomyces anomalus (LV-6),
Pichia kudriavzevii (LV-8), Kodamaea ohmeri (LV-9) and Trichosporon asahii (LV-10). A pre-screening of
the strains for probiotic use was based on their ability to agglutinate pathogenic micro-organisms. These
yeast strains were characterized for specific growth rate, duplication time, their cell surface hydrophobicity,
medium acidification, resistance to low pH (2.0, 2.5 and 3.0) and concentrations of bile salts (0.3% and
0.6%). The isolate of W. anomalus (LV-6) had the highest agglutinating and adherence capacity, a growth
rate of 2.07 _ 108 cfu/mL in 24 h at 30 _C, decreasing the medium pH from 6.5 to 5.23, a 25% hydrophobicity
and an elevated capacity to grow under stress conditions.
Conclusions: W. anomalus strain LV-6 showed the best characteristics for use as a probiotic candidate.
Significance and impact of the Study:
The data from this study helped in choosing a probiotic candidate from yeast to use in broiler
production.
The objective of this study was to isolate and identify yeast strains from broilers excreta and to evaluatein vitro their potential for probiotic use in animal production.Methods and results: Nine yeast strains were isolated and presumptively pre-identified by biochemicalassays. These isolates were grouped in six different molecular profiles using PCR-fingerprinting technique.Each profile was identified by sequencing of the D1/D2 domains of the large subunit of the 26SrRNA gene. These yeasts were identified as: Trichosporon sp. (LV-2), Wickerhamomyces anomalus (LV-6),Pichia kudriavzevii (LV-8), Kodamaea ohmeri (LV-9) and Trichosporon asahii (LV-10). A pre-screening ofthe strains for probiotic use was based on their ability to agglutinate pathogenic micro-organisms. Theseyeast strains were characterized for specific growth rate, duplication time, their cell surface hydrophobicity,medium acidification, resistance to low pH (2.0, 2.5 and 3.0) and concentrations of bile salts (0.3% and0.6%). The isolate of W. anomalus (LV-6) had the highest agglutinating and adherence capacity, a growthrate of 2.07 _ 108 cfu/mL in 24 h at 30 _C, decreasing the medium pH from 6.5 to 5.23, a 25% hydrophobicityand an elevated capacity to grow under stress conditions.Conclusions: W. anomalus strain LV-6 showed the best characteristics for use as a probiotic candidate.Significance and impact of the Study:The data from this study helped in choosing a probiotic candidate from yeast to use in broiler
production.
การแปล กรุณารอสักครู่..
