APX was measured following the methods of Nakano and Asada [29] by monitoring the rate of ascorbate oxidation at 290 nm
(E = 2.8 mM cm−1). The reaction mixture contained 25 mM phosphate buffer (pH 7.0), 0.1 mM EDTA, 100 mM H2O2, 0.25 mM ascorbate (AsA), and the enzyme aliquot.