Somatic embryos were treated with four different concentrations of colchicine (0, 0.3, 0.5, 1.0, %) in liquid MS medium supplemented with 0.22 mg/l zeatin, and shaken (60 rpm) at 25 0C in darkness for 48, 72 or 96 hours
Somatic embryos were treated with four different concentrations of colchicine (0, 0.3, 0.5, 1.0, %) in liquid MS medium supplemented with 0.22 mg/l zeatin, and shaken (60 rpm) at 25 0C in darkness for 48, 72 or 96 hours