In the present study a wide spectral antibacterial activity has been recorded in almost all the extracts. On comparison the maximum zone of inhibition was obtained for the crude ink L.duvauceli than the S.pharaonis which indicated the presence of potent antimicrobial compounds in them. But after partial purification and fractionation the antimicrobial activity was higher in the 80% fractionated S.pharaonis ink. So L.duvauceli and S.pharaonis would be a good source of antimicrobial agents and would replace the existing inadequate and cost effective antibiotics