In hatchery C, ten juveniles were collected from an earthen pond
and euthanised via medullar sectioning. Their whole body surfaces
were subsequently scraped, and the collected mucus was deposited
on a glass slide. The gills (all arches) were collected separately
from the body mucus and deposited between a slide and cover slip.
This material was also analysed fresh under an optical light
microscope (400×).