After the saccharification time of 48 h, the glucose syrup was
filtered and evaporated in an oven at 60 °C to give sugar concentrations
of 35–40% (w/v). The glucose syrup was adjusted to pH 7.5 and
kept in a thermostatic water bath at 60 °C and after attaining the
equilibrium temperature, Sweetzyme-T (500 mg for cassava and
300 mg for sweet potato with an activity of 508 Glucose Isomerase
Novo Units per gram) was added and incubated at 60 °C for 48 h.
Aliquots were drawn from each treatment and the fructose content
was estimated by the cysteine-carbazole method