Third method of titration, in terms of the measurement procedure, is similar to the first method (Titration I). However, the calculations are made based on the position of the first point on the titration curve, and not based on the difference between the position of the first and second inflection point as in method “Titration I”. Figure 4 shows a titration curve for chitosan solution using method of Titration III. The first maximum corresponds to neutralization of HCl, the second to the amount of NaOH needed for reaction with H+ on