The alkali-catalyzed transesterification reaction was performed using different alcohol to oil ratios and various amounts of catalyst. The mixture was vigorously stirred and refluxed on hot plate and temperature was controlled at 60°C for 20min.
The reaction mixture was kept for settling and separated to biodiesel and glycerol for further use in the study. Table 1 shows a summary of experimental plans used and yield of biodiesel product throughout this study.