After several cycles of operation (approximately 30 cycles) under the above-mentioned conditions, the behavior and performance of the culture stabilized and therefore bacteria that represented the ‘enriched’ culture were isolated. Bacterial isolates were obtained by subsequent dilutions and further streaking on basal medium (BM) agar plates. Medium’s composition was the same as basal synthetic medium (as described above) including 15 g/L agar.
The plates were incubated at 28 ◦C for 48 h and colonies from the plates were picked and further purified