The sample 15 g was blended (15 min) in 50 ml of acetonitrileewater (45:05, v/v), using high speed blending and then the extract was filtered through filter paper.
About 5 ml of the filtrate was mixed with 50 ml of phosphate buffer saline (PBS) and filtered through a glass microfiber.
Then 10 ml of the filtrate was passed through immunoaffinity columns.