The Trichoderma spp. were grown on Molasses-Soy Medium(MSM) by inoculation of 5 ml of conidial suspension from sporulating cultures on PDA slants to 250 ml of medium in 500-ml Erlenmeyer
flasks. Inoculated flasks were kept in shaking incubator (make Amerex Instruments Inc., USA) for 3 days at 200 rpm and culture flasks were removed and poured in to the sterile plastic box with dimensions of 36x 25 x 5 cm. The poured molasses-soya medium is spread uniformly in the tray maintained a depth of 5 mm. The trays with culture were incubated at 25°C for 3 days for profuse conidia production on the surface. The six-day-old dry fungal mat was ground to fine powder.
Dry conidial powder was mixed with mineral oil (density–0.838 g/ml
at 25°C, viscosity index–102) at 1:3 ratio, stabilizers were added and sedimentation rate or buoyancy was recorded accordingly. The SC formulation was packed in High Density Polyethylene (HDPE) bottles